Workshop: CRISPR-Cas9 Genome Editing Bootcamp

Recorded On: 10/15/2019

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Genome-editing tools, particularly CRISPR-Cas9, are becoming integral to the investigation of human genetics. This practical, hands-on workshop is intended for anybody interested in learning how to use CRISPR-based technologies, including newer applications such as base editing, epigenome editing, and alternative CRISPR enzymes. During the workshop, along with short lectures to cover basic concepts, participants will engage in active-learning, team-based exercises to learn online tools to design CRISPR reagents, common pitfalls to avoid, and interpretation of data from genome-editing experiments (requiring participants to bring their own WiFi-capable laptops). The initial part of the workshop is geared towards beginners rather than those with experience with genome editing, though all are welcome.


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Workshop Introduction & Context
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Exercise 1 & Discussion: Which exon to target to knock out each gene?
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More Details on Gene Editing
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Exercise 2 & Discussion: Candidate Guide RNA
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Assessing Efficacy
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Exercise 3 & Discussion: Electropherogram
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Knock In Variants Discussion
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Exercise 4 & Discussion: Knock In Variant
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