Emerging Laboratory Technologies SIG Seminar

Constellation: The Next Generation of Whole Genome Sequencing (WGS)

Ali Crawford is a Sr. Director at Illumina. In this seminar, Ali Crawford, PhD will present on Constellation mapped reads, a novel on flow cell whole genome assay. The Constellation mapped reads workflow moves typical DNA library prep directly onto the flow cell, where DNA is tagmented and sequenced in nanowells on the flow cell surface. Using information from neighboring nanowells, many additional insights can be derived with Constellation as compared to traditional short read whole genome sequencing. This workflow can be utilized for any researcher who is interested in additional insights from Illumina whole genome sequencing including haplotype information, improved callability in high homologous regions, and improved structural variant calling and visualization. This presentation will cover how these genomes compare to other sequencing technologies and ways to interpret these new outputs.

Overview of Presentation

• Overview of the Constellation technology
• Review of data outputs and benchmarks to other technology
• Specific examples of Constellation specific insights and how to interpret them